ACVR1 Knockout cell line (Hep G2)

ENCN
location： Home > Products > KO Cell Lines > ACVR1 Knockout cell line (Hep G2)

ACVR1 Knockout cell line (Hep G2) Order

ACVR1 Knockout cell line (Hep G2)
Catalog#
YKO-H1102
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
ACVR1 Knockout cell line (Hep G2)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
ACVR1 Knockout cell line（Hep G2）
Cell Morphology
Epithelial-like, adherent
Passage ratio
1:2~1:4
Species
Human
Gene Name
ACVR1
Gene ID
90
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Culture method
90% DMEM+10% FBS
Accession
CVCL_E0TI(Cellosaurus)
ExPASy Cellosaurus Database
Is a valuable online resource that compiles detailed information on over 100,000 cell lines from reputable cell banks such as ATCC, ECACC, and DSMZ. The database provides information including descriptions, origins, and characteristics of the cell lines, with broad reference value and scientific reliability. Additionally, the database provides information on cell line authentication, STR profiles, and potential contamination risks, helping researchers ensure the trustworthiness of the cell lines used in their experiments.
Target region
Knockout region: Exon 4 (based on transcript ACVR1-207)
Validation: PCR+Sanger Sequencing
ACVR1 Knockout cell line (Hep G2) Target region
STR Profile
Click to download the STR appraisal report
Loci STR Info (Sample Cell)
Sample Cell Line:Hep G2		 STR Info (Cell bank)
Cell Line:GS-HepG2
Allele1Allele2Allele3Allele1Allele2Allele3
D5S818 11121112
D13S317 913913
D7S820 10101010
D16S539 12121212
VWA 17171717
TH01 9999
AMEL XYXY
TPOX 8989
CSF1PO 10111011
D12S391 2125
FGA 2225
D2S1338 1920
D21S11 2931
D18S51 1314
D8S1179 1516
D3S1358 1516
D6S1043 1313
PENTAE 1520
D19S433 15.215.2
PENTAD 913
D1S1656 1112
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the ACVR1 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the Hep G2 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, ACVR1 gene knockout ACVR1 Knockout cell line（Hep G2） will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
ACVR1 Knockout cell line (Hep G2)
ACVR1 Knockout cell line (Hep G2)
Catalog#
YKO-H1102
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

Related Popular Products

Wild-type Cell Lines

Gene Knockout kit

EZ-cryo™ cell freezing medium

KO Cell Lines

  • ACVR1 Knockout cell line (Huh-7)

    ACVR1 Knockout cell line (Huh-7)
      inquiry   More details

    Organism: Human
    Gene Name: ACVR1
    Gene ID: 90
    Size: 1*10^6
    Catalog#: YKO-H1147
    YKO-H1147(HuH-7)
  • ACVR1 Knockout cell line (HeLa)

    ACVR1 Knockout cell line (HeLa)
      inquiry   More details

    Organism: Human
    Gene Name: ACVR1
    Gene ID: 90
    Size: 1*10^6
    Catalog#: YKO-HS3473
    YKO-HS3473(HeLa)
  • ACVR1 Knockout cell line (HCT 116)

    ACVR1 Knockout cell line (HCT 116)
      inquiry   More details

    Organism: Human
    Gene Name: ACVR1
    Gene ID: 90
    Size: 1*10^6
    Catalog#: YKO-HS2350
    YKO-HS2350(HCT 116)

Related Popular Products

Wild-type Cell Lines

Gene Knockout kit

EZ-cryo™ cell freezing medium

  • EZ-cryo™ Cell Freezing Medium

    EZ-cryo™ Cell Freezing Medium

    Price (USD): -;-
    Catalog#: YK-CR-50;YK-CR-100
    Size: 50ml;100ml
    Instruction: EZ-cryo™ Cell Freezing Medium
      inquiry   More details

KO Cell Lines

  • ACVR1 Knockout cell line (Huh-7)

    ACVR1 Knockout cell line (Huh-7)

    Organism: Human
    Gene Name: ACVR1
    Gene ID: 90
    Size: 1*10^6
    Catalog#: YKO-H1147
      inquiry   More details
  • ACVR1 Knockout cell line (HeLa)

    ACVR1 Knockout cell line (HeLa)

    Organism: Human
    Gene Name: ACVR1
    Gene ID: 90
    Size: 1*10^6
    Catalog#: YKO-HS3473
      inquiry   More details
  • ACVR1 Knockout cell line (HCT 116)

    ACVR1 Knockout cell line (HCT 116)

    Organism: Human
    Gene Name: ACVR1
    Gene ID: 90
    Size: 1*10^6
    Catalog#: YKO-HS2350
      inquiry   More details
×

Subscribe Us

By subscription, you consent to allow Ubigene Biosciences to store and process the information provided above to deliver the latest news, research spotlight, and promotions. You can unsubscribe from these communications at anytime.
WhatsApp: +86 153 6067 3248
TEL: +86 153 6067 3248 (Int'l)
US Toll free: 855 777 3210
EU Toll free: 800 3272 9252
Korea Toll free: 001 800 3272 9252

Gene Editing Services

EZ-editor™ Products

Copyright © 2024 Ubigene. All rights reserved.
×

Search documentation

Literature:

Name: *

Company: *

Telephone: *

Email:

Notes: After submitting the order, we will contact you as soon as possible.

request now